How do I work out the right amount of water to add for a CJC-1295 DAC dose?

Posted by fasted_scientist in Beginner Questions - 7 points, 6 comments.

I just got my first peptide vial – CJC-1295 DAC labelled 2mg. I’ve never reconstituted anything before and the instructions online are all over the place. I want to run a 100mcg dose per injection, twice a week, using an insulin syringe.

How much bacteriostatic water should I add to the 2mg powder so that each 100mcg is a simple, measurable volume? 5ml – does it matter as long as the math works out? Also, once mixed, how long can I keep it in the fridge before it degrades, and should I worry about bubbles when drawing it up?

Any tips for a total newbie on keeping things sterile and avoiding waste would be really helpful. Thanks in advance.

Comments

• sleep_coastal: The math is what matters but pick a volume that gives you a comfortable number on the syringe. 2mg is 2000mcg. 1ml or 10 units on a standard insulin syringe. Clean and easy. 5ml works math-wise but that is a lot of liquid for a small vial and may not even fit. I usually go with 2ml for 2mg vials anecdotally. DAC conjugate is fairly stable once mixed, I would not push past 3-4 weeks in the fridge though. Flick bubbles gently, do not shake. Wipe the stopper every time.
• curious_scientist: Good call on 2ml. With 2ml bac water in a 2mg vial, 100mcg is 0.1ml, which is 10 units on a U-100 insulin syringe. Pretty clean number to draw. Just wanna flag one thing in your post though, you wrote "1ml or 10 units" and those are not the same thing. 1ml is 100 units on a standard insulin syringe. 10 units is 0.1ml. Easy mixup but could really throw someone off on their first reconstitution. Imo 2ml is the sweet spot for 2mg vials. Easy math, fits the vial, comfortable draw volume.
• student748: Cheers for laying that out, super helpful for a first timer. Just one thing i want to double check though, you wrote "1ml or 10 units" but on a U-100 insulin syringe 1ml is 100 units right? 1ml = 10 units for the 100mcg dose with 2ml bac water. For what it is worth just want to make sure i've got it straight before i actually draw anything up.
• fasted_scientist: Ngl you are spot on, I completely messed up that math in my head. I was thinking 10 units for the dose but you are right that 1ml is 100 units on those U-100 syringes. I would have massively underdosed myself if I had actually gone ahead with that. If I go with your 2ml suggestion for the 2mg vial, that makes each 100mcg dose exactly 10 units on the syringe, right? Just want to be 100% sure before I start poking myself.
• fasted_scientist: Cheers for the math breakdown, 2ml making it 10 units is spot on, that's clean enough for me. Just to be fair though, I don't think I wrote "1ml or 10 units" anywhere in my post, I was asking about 5ml. Might be mixing me up with another thread? No worries either way, the correction is solid info for anyone reading. One thing, you reckon 2ml degrades the peptide faster than a smaller volume, or does dilution not really affect shelf life once it's in the fridge?
• fasted_scientist: Cheers for that, the 2ml suggestion makes way more sense. I was worried 5ml might be a squeeze but reckoned bigger volume meant easier measuring. 10 units on the syringe is spot on for me, nice and simple. One thing, you said 3-4 weeks in the fridge. At twice a week dosing I'd be using about 20 doses from a 2mg vial, so that's roughly 10 weeks. Does that mean I'd be binning half the vial?

Community discussion - research and educational context only. Not medical advice.