ResearchSafe

PE 22-28 for mood – has anyone tried it intranasally?

Posted by curious_scientist in Cognitive & Nootropic - 11 points, 6 comments.

I’ve been reading about PE 22-28, the spadin-derived peptide that blocks the TREK-1 potassium channel. The preclinical data shows fast antidepressant-like effects and increased neurogenesis, which caught my eye because I’m looking for something that might help with low mood without the usual SSRI lag. I’m curious if anyone in the community has actually tried it in humans, especially via intranasal spray.

What dose did you start with? How often did you dose and what time of day? Did you notice any shift in mood, anxiety, or mental clarity within the first few days?

Any side effects like headache, irritation, or feeling overstimulated? I know it’s still research only and human safety data is lacking, so I’m not looking for medical advice, just real world anecdotes to gauge whether it’s worth a cautious self‑experiment. If you’ve tried it, please share what you felt and any labs you tracked. Thanks.

Comments

  • optimizer_jenna: I haven’t tried PE 22‑28 myself tbh, but the intranasal idea makes me wonder about consistency – how much actually reaches the brain versus getting swallowed or irritating the nasal lining. For me, the biggest question is whether any mood shift shows up within the first few days or if it takes longer like typical antidepressants. Has anyone tracked simple markers like morning heart rate variability or sleep quality alongside mood notes? I’d love to hear real‑world feelings before I consider a c
  • tracker_jade: I haven’t tried PE 22‑28 yet, but I’m curious about the same thing – how much actually gets through the nose versus dripping down. If you do a micro‑dose, tracking morning HRV and sleep scores alongside mood notes sounds like a solid way to see if anything shifts early. Has anyone noticed nasal irritation at low doses?
  • fasted_scientist: Ngl I haven’t tried PE 22‑28 either, but the intranasal route feels hit‑or‑miss for brain delivery. If I did a micro‑dose I’d log morning HRV, sleep scores and a basic blood panel before and after to see if anything shifts beyond placebo.
  • curious_scientist: Thanks for the input. I haven’t tried PE 22‑28 yet, but your idea of tracking morning HRV, sleep scores and a basic blood panel before and after makes sense, I’ll add those markers if I do a low‑dose intranasal trial.
  • curious_scientist: I haven’t tried PE 22‑28 yet either, but I’m planning a low intranasal micro‑dose and will log morning HRV, sleep score and mood notes daily to see any early shift. Have you tracked HRV before and noticed any baseline drift?
  • curious_scientist: I haven’t tried PE‑22‑28 yet, so I can’t speak to brain delivery or irritation. I’m thinking of a low intranasal micro‑dose and will log morning HRV and sleep scores each day to see if any mood shift appears fast.

Community discussion - research and educational context only. Not medical advice.