Phosphate Buffered Saline (Phosphate Buffered Saline (PBS))
A pH-balanced salt solution (PBS) used in research and reconstitution to keep peptides and proteins stable at physiological pH. It is commonly used as a neutral, isotonic diluent that will not damage sensitive compounds.
How it works
The phosphate buffer maintains a stable, near-physiological pH (~7.4) while the saline provides isotonic conditions, protecting peptides and proteins from pH-driven degradation during dissolution and handling.
Key facts
- Molecular weight: Buffered salt solution
- Half-life: N/A (solvent)
- Bioavailability: N/A (diluent)
- Storage: Room temperature or refrigerated per formulation; keep sterile.
Dosing overview
- Typical dose: Volume to reach target concentration
- Frequency: As needed to reconstitute
- Duration: Per vial use
- Route: Added to peptide/protein vial
Protocol notes
- Used to reconstitute peptides/proteins that need a stable, physiological pH.
- Add gently to the lyophilized powder and swirl; avoid vigorous shaking.
- Often preferred in research contexts and for pH-sensitive compounds.
Reported benefits
- Maintains stable physiological pH
- Isotonic and gentle on peptides/proteins
- Neutral research-grade diluent
Possible side effects
- Generally inert
- Contamination risk if unpreserved and reused
- Not interchangeable with preserved multi-use diluents
Research
- Buffered diluents preserve peptide stability (2014): pH-buffered isotonic solvents reduced degradation of pH-sensitive peptides and proteins during reconstitution and storage.
Compare Phosphate Buffered Saline
Research and educational reference only. Not medical advice.