ResearchSafe

Melatonin (Melatonin (N-Acetyl-5-Methoxytryptamine))

Category: Sleep & Recovery. Status: OTC supplement / hormone.

The primary hormone that signals darkness and regulates the sleep-wake cycle. Beyond sleep onset, biohackers use it for circadian alignment, jet lag, and its potent antioxidant and mitochondrial-protective effects.

How it works

Produced by the pineal gland in response to darkness, melatonin binds MT1/MT2 receptors to promote sleep onset and shift circadian timing. It also acts as a direct free-radical scavenger and supports mitochondrial antioxidant defenses.

Key facts

  • Molecular weight: 232.28 Da
  • Half-life: ~40-60 minutes (immediate release)
  • Bioavailability: Low and variable oral (~15%); sublingual improves onset
  • Storage: Room temperature, protect from light.

Dosing overview

  • Typical dose: 0.3-5 mg before bed
  • Frequency: Once nightly
  • Duration: As needed or ongoing
  • Route: Oral or sublingual

Protocol notes

  • For sleep, low doses (0.3-1 mg) are often as effective as higher doses with fewer next-day effects.
  • Take 30-60 minutes before the desired bedtime.
  • For jet lag, dose at the target-destination bedtime for several nights.

Reported benefits

  • Faster sleep onset
  • Circadian rhythm alignment
  • Jet lag relief
  • Potent antioxidant
  • Mitochondrial protection
  • Possible immune support

Possible side effects

  • Morning grogginess (high doses)
  • Vivid dreams
  • Headache
  • Mild dizziness
  • Daytime sleepiness if mistimed

Research

  • Melatonin for sleep onset and jet lag (2019): Meta-analyses found melatonin reduced sleep-onset latency and effectively mitigated jet lag symptoms.
  • Melatonin as an antioxidant (2020): Demonstrated direct free-radical scavenging and mitochondrial protection beyond its circadian role.

Compare Melatonin

Research and educational reference only. Not medical advice.