TB4-Frag (TBF) (Thymosin Beta-4 Fragment)
An active fragment of Thymosin Beta-4 (TB-500) containing the key actin-binding domain responsible for tissue repair signaling. Smaller molecular size may improve tissue penetration while retaining the healing properties of the full-length peptide.
How it works
Retains the actin-sequestering motif of full TB-4, promoting cell migration, angiogenesis, and reduction of inflammation at injury sites. The shorter sequence may offer improved bioavailability and targeted tissue penetration.
Key facts
- Molecular weight: ~1,200-1,800 Da (fragment)
- Half-life: ~2-4 hours
- Bioavailability: High subcutaneous absorption
- Storage: Lyophilized: room temperature. Reconstituted: refrigerate 2-8C.
Dosing overview
- Typical dose: 200-750 mcg per dose
- Frequency: Daily or every other day
- Duration: 4-8 weeks
- Route: Subcutaneous injection
Protocol notes
- Loading: 500-750 mcg daily for 2 weeks.
- Maintenance: 200-500 mcg every other day for 4-6 weeks.
- Often used for localized injury protocols.
Reported benefits
- Wound and tissue healing
- Reduced inflammation
- Potential improved tissue penetration vs full TB-4
- Support for tendon and muscle repair
- Angiogenesis promotion
Possible side effects
- Injection site irritation
- Mild headache (rare)
- Fatigue (transient)
Research
- Active domain mapping of Thymosin Beta-4 (2018): The central actin-binding domain (Ac-SDKP and LKKTET motifs) retains the majority of TB4 healing activity.
Community reviews of TB4-Frag (TBF)
- peptide_sarah rated it 5/5 - Injury recovery was noticeably faster: Started TB4-Frag (TBF) for a nagging tendon issue that was not responding to rest alone. Within about 2-3 weeks I noticed reduced pain during movement and better range of motion. By week 5-6 I was back to normal activity. No dramatic side effects. Some mild redness at the injection site occasionally and one day of fatigue early on. Otherwise very clean compound in my experience. My physical therapist commented that my progress seemed faster than expected for this type of injury. Cannot prove causation but the timeline matched up. I keep a vial on hand now for future recovery needs.
- careful_claire rated it 5/5 - My physio noticed the difference: Recovery protocol with TB4-Frag (TBF) for about 2 weeks. The injured area went from constant nagging pain to manageable discomfort within 2 weeks, then to essentially pain-free by week 5. What surprised me was the systemic benefit. Sleep improved, general achiness from training reduced, and my skin looked healthier. The healing peptides seem to work on multiple levels beyond just the target injury. Sourcing is the main challenge. Insist on third-party testing and proper reconstitution. I noticed results are better when I inject close to the injury site rather than just in the belly fat.
- n_of_one rated it 4/5 - Chronic issue finally improving after 2 months: Ran TB4-Frag (TBF) through a recovery phase after pushing too hard in training. The difference versus my last recovery attempt without it was clear. Less downtime, less residual stiffness, and I bounced back to full training about 2 weeks sooner than I expected. Protocol was straightforward, daily injections in the AM. Mild injection site reactions a couple times, nothing major. No systemic side effects that I could identify. I am convinced this shortened my recovery meaningfully. The caveat is always sourcing and the fact that this is still a gray area legally and medically. Do your researc
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Research and educational reference only. Not medical advice.